Anticorps Recombinant de lapin anti-Nephrin

Nephrin Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250064G12

N° de cat : 85845-1-PBS

Synonymes

NPHS1, CNF, NPHN, NPHS 1, Renal glomerulus-specific cell adhesion receptor

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Galerie de données de validation

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  WB analysis of mouse kidney using 85845-1-RR (same clone as 85845-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 85845-1-RR (Nephrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85845-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85845-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85845-1-RR against Human Nephrin were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85845-1-PBS cible Nephrin dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Nephrin Protéine recombinante Ag18994
• Nom complet: nephrosis 1, congenital, Finnish type (nephrin)
• Masse moléculaire calculée: 1241 aa, 135 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC156935
• Symbole du gène: Nephrin
• Identification du gène (NCBI): 4868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

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