Anticorps Polyclonal de lapin anti-Nesprin 3

Nesprin 3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 27132-1-AP

Synonymes

C14orf49, Nesprin 3

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Galerie de données de validation

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  WB analysis of C2C12 using 27132-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 27132-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 27132-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules C2C12, tissu splénique de rat, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

27132-1-AP cible Nesprin 3 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nesprin 3 Protéine recombinante Ag26002
• Nom complet: chromosome 14 open reading frame 49
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC146604
• Symbole du gène: Nesprin 3
• Identification du gène (NCBI): 161176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

Publications

Species	Application	Title
mouse	IF	Front Cell Dev Biol; SUN5 Interacting With Nesprin3 Plays an Essential Role in Sperm Head-to-Tail Linkage: Research on Sun5 Gene Knockout Mice.; Authors - Yunfei Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ning (Verified Customer) (01-28-2023)

Good antibody to prob nesprin 3.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

Nesprin 3 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:27132-1-AP)

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