Anticorps Monoclonal anti-Nesprin 3

Nesprin 3 Monoclonal Antibody for WB, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D1G12

N° de cat : 68420-1-PBS

Synonymes

1D1G12, C14orf139, C14orf49, KASH domain-containing protein 3, KASH3

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Galerie de données de validation

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  WB analysis using 68420-1-Ig (same clone as 68420-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 68420-1-Ig (same clone as 68420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68420-1-Ig (same clone as 68420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68420-1-PBS cible Nesprin 3 dans les applications de WB, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Nesprin 3 Protéine recombinante Ag26012
• Nom complet: chromosome 14 open reading frame 49
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC146604
• Symbole du gène: Nesprin 3
• Identification du gène (NCBI): 161176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

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