Anticorps Polyclonal de lapin anti-Nesprin1/Syne-1

Nesprin1/Syne-1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 31095-1-AP

Synonymes

SYNE1, MYNE1, Myne 1, Enaptin, CPG2

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Galerie de données de validation

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  WB analysis using 31095-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31095-1-AP (SYNE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules U2OS, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31095-1-AP cible Nesprin1/Syne-1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nesprin1/Syne-1 Protéine recombinante Ag34809
• Nom complet: spectrin repeat containing, nuclear envelope 1
• Poids moléculaire observé: 120 kDa, 367 kDa, 642 kDa, 1000 kDa
• Numéro d’acquisition GenBank: BC039121
• Symbole du gène: SYNE1
• Identification du gène (NCBI): 23345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYNE1, also known as spectrin repeat containing nuclear envelope protein 1. It is expressed in skeletal and smooth muscle, as well as peripheral blood lymphocytes, and localizes to the nuclear membrane. Mutations in the SYNE1 gene have been associated with several diseases, including autosomal recessive spinocerebellar ataxia type 8 (also referred to as autosomal recessive cerebellar ataxia type 1 or recessive ataxia of Beauce) and Emery-Dreifuss muscular dystrophy 4, autosomal dominant. The SYNE1 gene exhibits ubiquitous expression across various tissues, including the ovary and bone marrow. The molecular weight of the SYNE1 protein can vary depending on the specific isoform. For example, the full-length nesprin-1 isoform, also known as nesprin-1 giant, has 8797 amino acids and a molecular weight of approximately 1000 kDa. Another isoform, nesprin-1α2, has a molecular weight of about 120 kDa (PMID: 27782104). The protein has other isoforms with molecular weights of 380 kDa, 167 kDa and 642 kDa respectively.

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