Anticorps Monoclonal anti-Nestin

Nestin Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D11H2

N° de cat : 66259-1-PBS

Synonymes

NES, Nestin

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Galerie de données de validation

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  WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 66259-1-Ig (same clone as 66259-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 66259-1-Ig (same clone as 66259-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66259-1-PBS cible Nestin dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nestin
• Masse moléculaire calculée: 177 kDa
• Poids moléculaire observé: 150-170 kDa
• Numéro d’acquisition GenBank: NM_006617
• Symbole du gène: Nestin
• Identification du gène (NCBI): 10763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

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