Anticorps Polyclonal de lapin anti-Nestin

Nestin Polyclonal Antibody for IF/ICC, IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-19483

Synonymes

NES

---

Galerie de données de validation

• 

  IF Staining of human kidney using CL488-19483

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Nestin antibody (CL488-19483) at dilution of 1:200.

• 

  IF Staining of human dental pulp stem cells using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp stem cells using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) at dilution 1:250 and CoraLite 594 Iba1 antibody (CL594-10904, in red) at dilution 1:250. DAPI in blue. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

• 

  IF Staining of human dental pulp stem cells using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp stem cells using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) at dilution 1:250 and CoraLite 594 Iba1 antibody (CL594-10904, in blue) at dilution 1:250. DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

• 

  IF Staining of human dental pulp-derived neuronal spheroid using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp-derived neuronal spheroid using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) and F-actin (in red). DAPI in blue. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

• 

  IF Staining of human dental pulp-derived neuronal spheroids using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp-derived neuronal spheroids using CoraLite Plus 488 Nestin antibody (CL488-19483, in blue) and F-actin (in yellow). DAPI in purple. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

• 

  IF Staining of human dental pulp stem cells using CL488-19483

  Immunofluorescent analysis of fixed human dental pulp stem cells using CoraLite Plus 488 Nestin antibody (CL488-19483, in green) at dilution 1:250 and CoraLite 594 Iba1 antibody (CL594-10904, in blue) at dilution 1:250. DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

---

Applications testées

• Résultats positifs en IF-P: tissu rénal humain,
• Résultats positifs en IF/ICC: human dental pulp stem cells, human dental pulp-derived neuronal spheroids

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-19483 cible Nestin dans les applications de IF/ICC, IF-P et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nestin
• Masse moléculaire calculée: 177 kDa
• Poids moléculaire observé: 170-180 kDa
• Numéro d’acquisition GenBank: NM_006617
• Symbole du gène: Nestin
• Identification du gène (NCBI): 10763
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

{{ptg:RelatedPrimaryAntibodies}}