Anticorps Polyclonal de lapin anti-NeuN

NeuN Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 23060-1-AP

Synonymes

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Galerie de données de validation

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  WB analysis of mouse brain using 23060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 23060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 23060-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 23060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23060-1-AP (NeuN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse brain using 23060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23060-1-AP (NeuN Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules 3T3-L1
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 8 publications below

Informations sur le produit

23060-1-AP cible NeuN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Chèvre, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NeuN Protéine recombinante Ag19347
• Nom complet: hexaribonucleotide binding protein 3
• Poids moléculaire observé: 38-50 kDa
• Numéro d’acquisition GenBank: BC093713
• Symbole du gène: NeuN
• Identification du gène (NCBI): 146713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events.

Publications

Species	Application	Title
goat	IF	Cell Cycle; Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.; Authors - Zhimin Wang
hamster	IHC	Neurotherapeutics; PMID: 28083805; Authors - Syed Zahid Ali Shah
rat	IF	Exp Cell Res; CEBPD aggravates apoptosis and oxidative stress of neuron after ischemic stroke by Nrf2/HO-1 pathway; Authors - Nan Chen
rat	WB,IF	Neurochem Int; Tert-butylhydroquinone post-treatment attenuates neonatal hypoxic-ischemic brain damage in rats.; Authors - Juan Zhang
mouse	IF	J Stroke Cerebrovasc Dis; An Increase of Sigma-1 Receptor in the Penumbra Neuron after Acute Ischemic Stroke.; Authors - Xiaotian Zhang
human	IHC	Pediatr Surg Int; Pathological changes of interstitial cells of Cajal and ganglion cells in the segment of resected bowel in Hirschsprung's disease.; Authors - Xuyong Chen

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