Anticorps Recombinant de lapin anti-NeuN

NeuN Recombinant Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241461G7

N° de cat : 84401-4-PBS

Synonymes

241461G7, Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen

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Galerie de données de validation

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  WB analysis using 84401-4-RR (same clone as 84401-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

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  WB analysis using 84401-4-RR (same clone as 84401-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84401-4-RR (NeuN antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 84401-4-RR (same clone as 84401-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 84401-4-RR (same clone as 84401-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84401-4-RR (same clone as 84401-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 84401-4-RR (same clone as 84401-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84401-4-RR (same clone as 84401-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84401-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84401-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84401-4-RR against Human NeuN were performed. The affinity constant is 0.986 nM.

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Informations sur le produit

84401-4-PBS cible NeuN dans les applications de WB, IHC, IF-P, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NeuN Protéine recombinante Ag25689
• Nom complet: hexaribonucleotide binding protein 3
• Poids moléculaire observé: 46-52 kDa
• Numéro d’acquisition GenBank: NM_001082575
• Symbole du gène: NeuN
• Identification du gène (NCBI): 146713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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