Anticorps Polyclonal de lapin anti-NeuN

NeuN Polyclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

N° de cat : CL555-26975

Synonymes

cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

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Galerie de données de validation

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  IF Staining of mouse brain using CL555-26975

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®555 NeuN antibody (CL555-26975) at dilution of 1:200.

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  FC experiment of U-87 MG using CL555-26975

  1X10^6 U-87 MG cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human NeuN (CL555-26975) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules MG U-87
• Résultats positifs en cytométrie: cellules MG U-87

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-26975 cible NeuN dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NeuN Protéine recombinante Ag25689
• Nom complet: hexaribonucleotide binding protein 3
• Poids moléculaire observé: 46-52 kDa
• Numéro d’acquisition GenBank: NM_001082575
• Symbole du gène: NeuN
• Identification du gène (NCBI): 146713
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).

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