Anticorps Monoclonal anti-NeuN

NeuN Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF-P

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3A4C1

N° de cat : CL594-66836

Synonymes

3A4C1, Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen

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Galerie de données de validation

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  IF Staining of mouse cerebellum using CL594-66836

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1 ) at dilution of 1:200.

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  IF Staining of mouse cerebellum using CL594-66836

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1 ) at dilution of 1:200.

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  IF Staining of rat brain using CL594-66836

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1 ) at dilution of 1:400, CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Magenta), CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6, golden), CoraLite® Plus 488 MBP antibody (CL488-10458, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using CL594-66836

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1 ) at dilution of 1:400, CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Magenta), CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6, golden), CoraLite® Plus 488 MBP antibody (CL488-10458, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IF-P: tissu de cervelet de souris, tissu cérébral de rat

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66836 cible NeuN dans les applications de IF-P et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NeuN Protéine recombinante Ag28016
• Nom complet: hexaribonucleotide binding protein 3
• Numéro d’acquisition GenBank: NM_001082575
• Symbole du gène: NeuN
• Identification du gène (NCBI): 146713
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

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