Anticorps Polyclonal de lapin anti-Neurofibromin 1

• Phare
• Validé par KD/KO

Neurofibromin 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 27249-1-AP

Synonymes

Neurofibromin, NF1, NF 1, NF-1

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Galerie de données de validation

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  WB analysis using 27249-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27249-1-AP (Neurofibromin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27249-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27249-1-AP (Neurofibromin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 27249-1-AP

  IP result of anti-Neurofibromin 1 (IP:27249-1-AP, 4ug; Detection:27249-1-AP 1:1000) with HEK-293 cells lysate 1800 ug.

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  IHC staining of mouse brain using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27249-1-AP (Neurofibromin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27249-1-AP (Neurofibromin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 27249-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27249-1-AP (Neurofibromin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293T, cellules HeLa
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

27249-1-AP cible Neurofibromin 1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Neurofibromin 1 Protéine recombinante Ag25799
• Nom complet: neurofibromin 1
• Masse moléculaire calculée: 319 kDa
• Poids moléculaire observé: 250-280 kDa
• Numéro d’acquisition GenBank: M60915
• Symbole du gène: Neurofibromin 1
• Identification du gène (NCBI): 4763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).

Publications

Species	Application	Title
		Nat Commun; Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma; Authors - Perla Pucci
human	WB	Clin Transl Med; IGF2BP3 mediates the mRNA degradation of NF1 to promote triple-negative breast cancer progression via an m6A-dependent manner; Authors - Xu Zhang
human	WB,IHC	Gynecol Oncol; Survey of NF1 inactivation by surrogate immunohistochemistry in ovarian carcinomas; Authors - Martin Köbel
human	WB,IP	Front Genet; Characterization of Two Loss-of-Function NF1 Variants in Chinese Patients and Potential Molecular Interpretations of Phenotypes.; Authors - Tingting Zhang
human	WB	Mol Genet Genomic Med; A novel mutation in NF1 gene of patient with Neurofibromatosis type 1: A case report and functional study.; Authors - Tingting Zhang
human	WB,IHC,IF	J Transl Med; The oncogenic role of NF1 in gallbladder cancer through regulation of YAP1 stability by direct interaction with YAP1; Authors - Lingxiao Zhang ; KD Validated

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