Anticorps Recombinant de lapin anti-Neurofibromin

Neurofibromin Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240540F2

N° de cat : 83620-5-PBS

Synonymes

NF1, 240540F2, neurofibromin 1, NF 1, NF-1

---

Galerie de données de validation

• 

  WB analysis using 83620-5-RR (same clone as 83620-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 83620-5-RR (same clone as 83620-5-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83620-5-RR (same clone as 83620-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00570-2

  Cytometric bead array standard curve of MP00570-2, Neurofibromin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83620-5-PBS. Detection antibody: 83620-4-PBS. Standard: Ag25799. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 83620-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 83620-5-PBS against Human Neurofibromin 1 was performed. The affinity constant is 68.2 pM.

• 

  Affinity and Kinetic Characterization of 83620-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83620-5-RR against Human Neurofibromin 1 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

83620-5-PBS cible Neurofibromin dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Neurofibromin Protéine recombinante Ag25799
• Nom complet: neurofibromin 1
• Masse moléculaire calculée: 319 kDa
• Poids moléculaire observé: 250-280 kDa
• Numéro d’acquisition GenBank: M60915
• Symbole du gène: Neurofibromin 1
• Identification du gène (NCBI): 4763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).

{{ptg:RelatedPrimaryAntibodies}}