Anticorps Monoclonal anti-Neuropilin 1/CD304

• Phare
• Validé par KD/KO

Neuropilin 1/CD304 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H3F6

N° de cat : 60067-1-PBS

Synonymes

Neuropilin 1, NRP1, 2H3F6, BDCA4, CD304

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Galerie de données de validation

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  WB analysis using 60067-1-Ig (same clone as 60067-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60067-1-Ig (same clone as 60067-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit heart using 60067-1-Ig (same clone as 60067-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-231 using 60067-1-Ig (same clone as 60067-1-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60067-1-Ig (same clone as 60067-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 60067-1-Ig (same clone as 60067-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60067-1-Ig (same clone as 60067-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 60067-1-Ig (same clone as 60067-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 60067-1-Ig (same clone as 60067-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  IF Staining of human embronic stem cells using 60067-1-Ig (same clone as 60067-1-PBS)

  Confocal immunofluorescent analysis of human embronic stem cells with 60067-1-Ig at dilution of 1:100. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml. This photo is the merge of PE and DAPI. (40X). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 60067-1-Ig (same clone as 60067-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60067-1-Ig(Neuropilin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60067-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50479-1

  Cytometric bead array standard curve of MP50479-1, NRP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60067-1-PBS. Detection antibody: 60067-2-PBS. Standard:Ag0931. Range: 0.391-100 ng/mL.

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Informations sur le produit

60067-1-PBS cible Neuropilin 1/CD304 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc

• Réactivité: Humain, Lapin, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Neuropilin 1/CD304 Protéine recombinante Ag0931
• Nom complet: neuropilin 1
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC007737
• Symbole du gène: Neuropilin 1
• Identification du gène (NCBI): 8829
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

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