Anticorps Monoclonal anti-Neuroserpin

Neuroserpin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G4C6

N° de cat : 66997-1-PBS

Synonymes

Serpin I1, 1G4C6, Peptidase inhibitor 12, PI 12, PI12

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Galerie de données de validation

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  WB analysis of pig brain using 66997-1-Ig (same clone as 66997-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66997-1-Ig (SERPINI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 66997-1-Ig (same clone as 66997-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Neuroserpin antibody (66997-1-Ig, Clone: 1G4C6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66997-1-PBS cible Neuroserpin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Neuroserpin Protéine recombinante Ag28636
• Nom complet: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• Masse moléculaire calculée: 410 aa, 47 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC018043
• Symbole du gène: Neuroserpin
• Identification du gène (NCBI): 5274
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.

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