Anticorps Monoclonal anti-NeutraControl IFN-gamma

NeutraControl IFN-gamma Monoclonal Antibody for ELISA, Non-Neutralization

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Conjugaison

Non conjugué

CloneNo.

2F11G6

N° de cat : 69507-1-PBS

Synonymes

NeutraControl IFN gamma, IFNG, 2F11G6, IFG, IFN gamma

---

Galerie de données de validation

• 

  Non-Neutralization experiment of HT-29 cell using 69507-1-Ig (same clone as 69507-1-PBS)

  Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69507-1-PBS in a different storage buffer formulation.

• 

  ELISA experiment of Recombinant protein using 69507-1-Ig (same clone as 69507-1-PBS)

  Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69507-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

69507-1-PBS cible IFN-gamma dans les applications de et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human Humankine IFN-gamma protein HZ-1301
• Nom complet: IFN gamma
• Symbole du gène: IFNG
• Identification du gène (NCBI): 3458
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS Only
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody is a neutralizing control antibody for IFN gamma, the immunogen is the same as Neutrakine 69007-1-Ig but could not neutralize human IFN gamma.

{{ptg:RelatedPrimaryAntibodies}}