Anticorps Monoclonal anti-NeutraKine® GM-CSF

NeutraKine® GM-CSF Monoclonal Antibody for Neutralization, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Neutralization, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E10E7

N° de cat : 69003-1-PBS

Synonymes

CSF2, GM-CSF, CSF 2, CSF, Colony-stimulating factor

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP50067-1

  Sandwich ELISA standard curve of MP50067-1, GM-CSF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69003-1-PBS. Detection antibody: 68702-1-PBS. Standard: Eg0189. Range: 7.8-500 pg/mL.

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  Neutralization experiment of NeutraKine® GM-CSF using 69003-1-Ig (same clone as 69003-1-PBS)

  Recombinant human GM-CSF (Cat.NO. HZ-1002) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human GM-CSF (0.2 ng/mL HZ-1002) is neutralized by mouse anti-human GM-CSF monoclonal antibody 69003-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1.5-4 μg/mL This data was developed using the same antibody clone with 69003-1-PBS in a different storage buffer formulation.

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Informations sur le produit

69003-1-PBS cible NeutraKine® GM-CSF dans les applications de Neutralization, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human Humankine GM-CSF protein HZ-1002
• Nom complet: colony stimulating factor 2 (granulocyte-macrophage)
• Symbole du gène: GM-CSF
• Identification du gène (NCBI): 1437
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

This antibody can be used to neutralize the bioactivity of GM-CSF.

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