Anticorps Monoclonal anti-NeutraKine® IFN Alpha 2A

NeutraKine® IFN Alpha 2A Monoclonal Antibody for Neutralization, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Neutralization, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

2H8G1

N° de cat : 69008-1-PBS

Synonymes

IFN alpha 2, IFNA2, 2H8G1, IFN alpha 2A, IFN alpha2

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP50005-1

  Sandwich ELISA standard curve of MP50005-1, IFNA2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69008-1-PBS. Detection antibody: 68665-1-PBS. Standard: HZ-1066. Range: 7.8-500 pg/mL.

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  Sample test of MP50005-1

  Serum of six individual healthy human donors was measured. The IFN alpha 2 concentration of detected samples was determined to be 66.5 pg/mL with a range of 29.0-185.5 pg/mL.

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  Sample test of MP50005-1

  Human peripheral blood mononuclear cells (PBMC) was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were unstimulated or stimulated with 10 μg/mL poly I:C and Lipofectamine 2000 (LF2K) for 1 day. An aliquot of the cell culture supernatant was removed, the mean IFN alpha 2 concentration was undetectable in unstimulated PBMC supernatant, 159.7 pg/mL in poly I:C and LF2K stimulated PBMC supernatant.

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  Neutralization experiment of NeutraKine® IFN Alpha 2A using 69008-1-Ig (same clone as 69008-1-PBS)

  Recombinant human IFN alpha 2A (Cat.NO. HZ-1066) inhibits the growth of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IFN alpha 2A (100 ng/mL HZ-1066) is neutralized by mouse anti-human IFN alpha 2A monoclonal antibody 69008-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 300-500 ng/mL. This data was developed using the same antibody clone with 69008-1-PBS in a different storage buffer formulation.

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Informations sur le produit

69008-1-PBS cible NeutraKine® IFN Alpha 2A dans les applications de Neutralization, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human Humankine IFN Alpha 2A protein HZ-1066
• Nom complet: interferon, alpha 2
• Symbole du gène: IFN alpha2
• Identification du gène (NCBI): 3440
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

Interferon alpha 2 is a type 1 interferon. Interferon alpha 2 is secreted by virus-infected cells and inhibits other cells from further infection. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761).

This antibody can be used to neutralize the bioactivity of Interferon alpha 2.

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