Anticorps Monoclonal anti-NeutraKine® IL-10

NeutraKine® IL-10 Monoclonal Antibody for IHC, Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

1E4F5

N° de cat : 69018-1-PBS

Synonymes

IL-10, IL-10,IL10, 1E4F5, CSIF, IL 10

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP50035-1

  Sandwich ELISA standard curve of MP50035-1, IL-10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69018-1-PBS. Detection antibody: 69018-2-PBS. Standard: HZ-1145. Range: 15.6-1000 pg/mL.

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  Sample test of MP50035-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 day or 5 days. For the PBMC supernatant cultured for 1 day, the mean IL-10 concentration was determined to be 431.4 pg/mL in unstimulated PBMC supernatant, 1,534.2 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean IL-10 concentration was determined to be 225.9 pg/mL in unstimulated PBMC supernatant, 634.8 pg/mL in PHA stimulated PBMC supernatant.

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  Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

  Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 50-200 ng/mL. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

  Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 69018-1-Ig (same clone as 69018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 69018-1-Ig (same clone as 69018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  ELISA experiment of Recombinant protein using 69018-1-Ig (same clone as 69018-1-PBS)

  Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 69018-1-Ig

  iolayer interferometry (BLl) kinetic assays of 69018-1-Ig against Human IL-10 were performed. The affinity constant is 0.377 nM.

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Informations sur le produit

69018-1-PBS cible NeutraKine® IL-10 dans les applications de IHC, Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human Humankine IL-10 protein HZ-1145
• Nom complet: interleukin 10
• Symbole du gène: IL-10
• Identification du gène (NCBI): 3586
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody can be used to neutralize the bioactivity of IL-10.

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