Anticorps Monoclonal anti-NeutraKine® IL-3

NeutraKine® IL-3 Monoclonal Antibody for Neutralization, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Neutralization, ELISA

Conjugaison

Non conjugué

CloneNo.

3C11G7

N° de cat : 69004-1-PBS

Synonymes

IL3, IL-3, 3C11G7, IL 3, Interleukin 3

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Galerie de données de validation

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  Neutralization experiment of NeutraKine® IL-3 using 69004-1-Ig (same clone as 69004-1-PBS)

  Recombinant human IL-3 (Cat.NO. HZ-1074) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-3 (10 ng/mL HZ-1074) is neutralized by mouse anti-human IL-3 monoclonal antibody 69004-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 40-70 ng/mL. This data was developed using the same antibody clone with 69004-1-PBS in a different storage buffer formulation.

• 

  Neutralization experiment of NeutraKine® IL-3 using 69004-1-Ig (same clone as 69004-1-PBS)

  Recombinant human IL-3 (Cat.NO. HZ-1074) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-3 (0.5 ng/mL HZ-1074) is neutralized by mouse anti-human IL-3 monoclonal antibody 69004-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.8-2.4 ng/mL. This data was developed using the same antibody clone with 69004-1-PBS in a different storage buffer formulation.

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Informations sur le produit

69004-1-PBS cible NeutraKine® IL-3 dans les applications de Neutralization, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human Humankine IL-3 protein HZ-1074
• Nom complet: interleukin 3 (colony-stimulating factor, multiple)
• Symbole du gène: IL-3
• Identification du gène (NCBI): 3562
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196).

This antibody can be used to neutralize the bioactivity of IL-3.

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