Anticorps Monoclonal anti-NeutraKine® TNF-alpha

NeutraKine® TNF-alpha Monoclonal Antibody for Neutralization, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

Neutralization, ELISA

Conjugaison

Non conjugué

CloneNo.

11H8A5

N° de cat : 69002-1-PBS

Synonymes

NeutraKine® TNF Alpha, TNF, TNF alpha, TNF-a, 11H8A5

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Galerie de données de validation

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  Neutralization experiment of NeutraKine® TNF-alpha using 69002-1-Ig (same clone as 69002-1-PBS)

  Recombinant human TNF alpha (Cat.NO. HZ-1014) inhibits the growth of L-929 cell line in the presence of actinomycin D in a dose dependent manner (blue curve, refer to bottom X-left Y). The activity elicited by recombinant human TNF alpha (0.05 ng/mL HZ-1014) is neutralized by mouse anti-human TNF alpha monoclonal antibody 69002-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 10-50 ng/mL. The NeutraControl mouse anti-human TNF alpha monoclonal antibody 69502-1-Ig, generated from the same immunogen, could recognize human TNF alpha, but could not neutralize human TNF alpha at the range of 0.128-10000ng/mL (green curve, refer to top X-right Y). This data was developed using the same antibody clone with 69002-1-PBS in a different storage buffer formulation.

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  ELISA experiment of Recombinant protein using 69002-1-Ig (same clone as 69002-1-PBS)

  Indirect ELISA was carried out by coating recombinant Human TNF Alpha (Cat.NO. HZ-1014) at 70 ng/well followed by blocking and adding serial diluted TNF Alpha antibody 69002-1-Ig and 69502-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69002-1-PBS in a different storage buffer formulation.

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Informations sur le produit

69002-1-PBS cible NeutraKine® TNF-alpha dans les applications de Neutralization, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human Humankine TNF-alpha protein HZ-1014
• Nom complet: tumor necrosis factor (TNF superfamily, member 2)
• Symbole du gène: TNF-alpha
• Identification du gène (NCBI): 7124
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

This antibody can be used to neutralize the bioactivity of TNF alpha.

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