Anticorps Monoclonal anti-NeutraKine®Thrombopoietin

NeutraKine®Thrombopoietin Monoclonal Antibody for Neutralization, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Neutralization, ELISA

Conjugaison

Non conjugué

CloneNo.

2H8G1

N° de cat : 69028-1-Ig

Synonymes

C mpl ligand, MGDF, MKCSF, ML, MPLLG, THPO, thrombopoietin, TPO

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Galerie de données de validation

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  Neutralization experiment of NeutraKine®Thrombopoietin using 69028-1-Ig

  Recombinant human Thrombopoietin (Cat.NO. HZ-1248) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human Thrombopoietin (30 ng/mL HZ-1248) is neutralized by mouse anti-human Thrombopoietin monoclonal antibody 69028-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.1-0.5μg/mL.

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  Neutralization experiment of NeutraKine®Thrombopoietin using 69028-1-Ig

  Recombinant human Thrombopoietin (Cat.NO. HZ-1248) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human Thrombopoietin (30 ng/mL HZ-1248) is neutralized by mouse anti-human Thrombopoietin monoclonal antibody 69028-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.1-0.5μg/mL.The NeutraControl mouse anti-human Thrombopoietin antibody 69528-1-Ig could recognize human Thrombopoietin but could not neutralize human Thrombopoietin acitivity in the range of 0.00122-20 μg/mL (green curve, refer to top X-right Y axis).

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  ELISA experiment of Recombinant protein using 69028-1-Ig

  Indirect ELISA was carried out by coating recombinant Human Thrombopoietin (Cat.NO. HZ-1248) at 70 ng/well followed by blocking and adding serial diluted Thrombopoietin antibody 69028-1-Ig and 69528-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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• Neutralization: Cellule TF-1

Informations sur le produit

69028-1-Ig cible NeutraKine®Thrombopoietin dans les applications de Neutralization, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human HumankineThrombopoietin protein HZ-1248
• Nom complet: thrombopoietin
• Symbole du gène: THPO
• Identification du gène (NCBI): 7066
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS stérile.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

This antibody can be used to neutralize the bioactivity of Thrombopoietin.