Anticorps Polyclonal de lapin anti-Nexilin

Nexilin Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27737-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 27737-1-AP (same clone as 27737-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27737-1-AP (Nexilin antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

27737-1-PBS cible Nexilin dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nexilin Protéine recombinante Ag26938
• Nom complet: nexilin (F actin binding protein)
• Masse moléculaire calculée: 675 aa, 81 kDa
• Poids moléculaire observé: 80-85 kDa
• Numéro d’acquisition GenBank: BC114444
• Symbole du gène: Nexilin
• Identification du gène (NCBI): 91624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nexilin, Also known as NEXN, NELIN, is a filamentous actin-binding protein that localizes to focal contacts and may be involved in cell adhesion and migration(PMID: 15823560). Nexilin is abundantly expressed in heart and skeletal muscle and has an essential role in the maintenance of Z line and sarcomere integrity(PMID: 12053183, 19881492). Mutations in Nexilin have been associated with cardiomyopathy dilated type 1CC (CMD1CC) and cardiomyopathy familial hypertrophic type 20 (CMH20)(PMID: 19881492, 20970104).

{{ptg:RelatedPrimaryAntibodies}}