Anticorps Polyclonal de lapin anti-Nicastrin

• Phare
• Validé par KD/KO

Nicastrin Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14071-1-PBS

Synonymes

UNQ1874/PRO4317, NCSTN, KIAA0253

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 14071-1-AP (same clone as 14071-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14071-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 14071-1-AP (same clone as 14071-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14071-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 14071-1-AP (same clone as 14071-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14071-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C6 using 14071-1-AP (same clone as 14071-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using Nicastrin antibody (14071-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14071-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14071-1-PBS cible Nicastrin dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nicastrin Protéine recombinante Ag5137
• Nom complet: nicastrin
• Masse moléculaire calculée: 709 aa, 78.4 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC047621
• Symbole du gène: Nicastrin
• Identification du gène (NCBI): 23385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease cleaves many type I membrane proteins including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytical processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).

{{ptg:RelatedPrimaryAntibodies}}