Anticorps Monoclonal anti-Ninein

• Phare
• Validé par KD/KO

Ninein Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

canin, Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B10A8

N° de cat : 67132-1-PBS

Synonymes

NIN, 1B10A8, Glycogen synthase kinase 3 beta-interacting protein, GSK3B interacting protein, GSK3B-interacting protein

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Galerie de données de validation

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  WB analysis using 67132-1-Ig (same clone as 67132-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67132-1-Ig (Ninein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 67132-1-Ig (same clone as 67132-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 67132-1-Ig (Ninein antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67132-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67132-1-PBS cible Ninein dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Ninein Protéine recombinante Ag14652
• Nom complet: ninein (GSK3B interacting protein)
• Masse moléculaire calculée: 2090 aa, 243 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC034708
• Symbole du gène: Ninein
• Identification du gène (NCBI): 51199
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.

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