Anticorps Polyclonal de lapin anti-NLRP3

NLRP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(43)

N° de cat : 30109-1-AP

Synonymes

AGTAVPRL, AII/AVP, Mmig1, MWS, NALP3

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Galerie de données de validation

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  WB analysis using 30109-1-AP

  Non-treated RAW 264.7 cells and LPS-treated RAW 264.7 cells lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (NLRP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (NLRP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (NLRP3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 30109-1-AP

  Rat spleen tissue was subjected to SDS PAGE, followed by western blot with 30109-1-AP (NLRP3 antibody) at a dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of RAW 264.7 using 30109-1-AP

  IP result of anti-NLRP3 (IP:30109-1-AP, 4ug; Detection:30109-1-AP 1:5000) with RAW 264.7 cells lysate 1680 ug.

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  IP experiment of RAW 264.7 using 30109-1-AP

  IP result of anti-Nlrp3 (IP:30109-1-AP, 4ug; Detection:30109-1-AP 1:4000) with RAW 264.7 cells lysate 1200 ug.

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  IHC staining of mouse liver using 30109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30109-1-AP (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 30109-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Nlrp3 antibody (30109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of RAW 264.7 using 30109-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using NLRP3 antibody (30109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7 traitées au LPS, cellules RAW 264.7, tissu splénique de rat
• Résultats positifs en IP: cellules RAW 264.7,
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 37 publications below
• IHC: See 8 publications below
• IF: See 12 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

30109-1-AP cible NLRP3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NLRP3 Protéine recombinante Ag32515
• Nom complet: NLR family, pyrin domain containing 3
• Masse moléculaire calculée: 118kd
• Poids moléculaire observé: 108-120 kDa
• Numéro d’acquisition GenBank: NM_145827
• Symbole du gène: Nlrp3
• Identification du gène (NCBI): 216799
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse NLRP3.

Publications

Species	Application	Title
mouse	IHC	Phytomedicine; ErTao decoction alleviates liver fibrosis by suppressing STING-mediated macrophages and NLRP3 inflammasome activation; Authors - Hui Jia
mouse	WB	J Agric Food Chem; Probiotic Combination of Limosilactobacillus fermentum HF07 and Lactococcus lactis HF08 Targeting Gut Microbiota-Secondary Bile Acid Metabolism Ameliorates Inflammation and Intestinal Barrier Dysfunction in Aging Colitis; Authors - Xiaolin Liu
mouse	WB	J Agric Food Chem; Ocotillol-Type Pseudoginsenoside-F11 Alleviates Lipopolysaccharide-Induced Acute Kidney Injury through Regulation of Macrophage Function by Suppressing the NF-κB/NLRP3/IL-1β Signaling Pathway; Authors - Yaru Wang
mouse	WB,IF	Int Immunopharmacol; Triggering receptor expressed on myeloid cells-1 aggravates obliterative bronchiolitis via enhancing the proinflammatory phenotype of macrophages; Authors - Junhao Wan
mouse	WB	Int Immunopharmacol; UCP2 knockout exacerbates sepsis-induced intestinal injury by promoting NLRP3-mediated pyroptosis; Authors - Bolun Huang
rat,mouse	WB,IHC,IF	Int Immunopharmacol; Therapeutic potential of quercitrin in intervertebral disc degeneration: Targeting pyroptosis and inflammation; Authors - Xinyu Wu

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