Anticorps Recombinant de lapin anti-NOV/CCN3

NOV/CCN3 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250054B2

N° de cat : 85731-1-PBS

Synonymes

CCN family member 3, Ccn3, Cellular communication network factor 3, Nephroblastoma-overexpressed gene protein homolog, Nov

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Galerie de données de validation

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  WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85731-1-PBS in a different storage buffer formulation.

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  WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85731-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85731-1-RR (same clone as 85731-1-PBS)

  Mouse brain tissue was subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85731-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02088-1

  Cytometric bead array standard curve of MP02088-1, MOUSE Nov/CCN3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85731-2-PBS. Detection antibody: 85731-1-PBS. Standard: Eg2856. Range: 1.563-200 ng/mL.

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Informations sur le produit

85731-1-PBS cible NOV/CCN3 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NOV/CCN3 Protéine recombinante Eg2856
• Nom complet: nephroblastoma overexpressed gene
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: NM_010930.4
• Symbole du gène: Nov
• Identification du gène (NCBI): 18133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCN family member 3 (CCN3), also known as Nephroblastoma overexpressed (NOV) protein, is a member of the CCN protein family (PMID: 1309586). CCN3 is predominantly expressed in the cytoplasm, nucleus, and extracellular space. As a subset of extracellular matrix proteins, it can directly bind to the integrin receptor family on the cell surface and participate in signal transduction between the cell surface and the extracellular matrix structure (PMID: 37378812). CCN3 exhibits anti-proliferative activity in normal cells and most tumor cells (PMID: 17340618). CCN3 co-localizes with connexin 43 (C×43) in the plasma membrane, and these two proteins physically interact to inhibit cell growth (PMID: 15213231). CCN3 can induce myofibroblast differentiation through the Notch pathway (PMID: 12050162). CCN can promote adhesion of endothelial cells, vascular smooth muscle cells, and fibroblasts through heparan sulfate proteoglycans (HSPG) (PMID: 30158025).

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