Anticorps Polyclonal de lapin anti-Nrf2

Nrf2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 33123-1-AP

Synonymes

Nfe2l2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor erythroid 2-related factor 2, Nuclear factor, erythroid derived 2, like 2

---

Galerie de données de validation

• 

  WB analysis using 33123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33123-1-AP (Nrf2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu de côlon de souris, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

33123-1-AP cible Nrf2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nrf2 Protéine recombinante Ag39859
• Nom complet: nuclear factor, erythroid derived 2, like 2
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 110 kDa, 75 kDa
• Numéro d’acquisition GenBank: NM_010902.4
• Symbole du gène: Nfe2l2
• Identification du gène (NCBI): 18024
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Publications

Species	Application	Title
mouse	WB	Food Funct; Ferulic acid identification in brown rice using untargeted metabolomics and network analysis and its effects in mitigating AFB1-induced hepatic damage; Authors - Qianqian Wang

{{ptg:RelatedPrimaryAntibodies}}