Anticorps Recombinant de lapin anti-Nucleoside phosphorylase

Nucleoside phosphorylase Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251603E11

N° de cat : 86651-1-RR

Synonymes

PNP, EC:2.4.2.1, Inosine phosphorylase, Inosine-guanosine phosphorylase, PRO1837

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Galerie de données de validation

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  WB analysis using 86651-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86651-1-RR (Nucleoside phosphorylase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 86651-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using NP antibody (86651-1-RR, Clone: 251603E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86651-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86651-1-RR against Human Nucleoside phosphorylase were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HL-60, cellules K-562, cellules NIH/3T3, cellules Raji, cellules U-937, tissu splénique de rat
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86651-1-RR cible Nucleoside phosphorylase dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Nucleoside phosphorylase Protéine recombinante Ag12507
• Nom complet: nucleoside phosphorylase
• Masse moléculaire calculée: 289 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC106074
• Symbole du gène: Purine nucleoside phosphorylase
• Identification du gène (NCBI): 4860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Purine nucleoside phosphorylases (PNP) is a ubiquitous enzyme of purine metabolism that functions in the salvage pathway, including even those of protozoan parasites, thus enabling the cells to utilize purine bases recovered from metabolized purine ribo- and deoxyribonucleosides to synthesize purine nucleotides (PMID: 11337031, 23332162). PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function. hPNP is widely distributed in various tissues and cells of the human body, with the highest activity in kidney, peripheral lymphocytes and granulocytes (PMID: 38701712).

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