Anticorps Polyclonal de lapin anti-Nurr1/NR4A2

Nurr1/NR4A2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 10975-2-PBS

Synonymes

NR4A2, NURR1, HZF 3, Immediate-early response protein NOT, NOT

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 10975-2-AP (same clone as 10975-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 10975-2-AP (same clone as 10975-2-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  IF Staining of Customer Sample using 10975-2-AP (same clone as 10975-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded Customer Sample customer using Nurr1/NR4A2 antibody (10975-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10975-2-AP (same clone as 10975-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Nurr1/NR4A2 antibody (10975-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10975-2-PBS cible Nurr1/NR4A2 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nurr1/NR4A2 Protéine recombinante Ag1418
• Nom complet: nuclear receptor subfamily 4, group A, member 2
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC009288
• Symbole du gène: Nurr1
• Identification du gène (NCBI): 4929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

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