Anticorps Monoclonal anti-Nurr1/NR4A2

• Phare
• Validé par KD/KO

Nurr1/NR4A2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H5B4

N° de cat : 66878-1-PBS

Synonymes

NR4A2, NURR1, 1H5B4, HZF 3, Immediate-early response protein NOT

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Galerie de données de validation

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  WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  WB analysis using 66878-1-Ig (same clone as 66878-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66878-1-Ig (same clone as 66878-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66878-1-Ig (same clone as 66878-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66878-1-Ig (same clone as 66878-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66878-1-Ig (same clone as 66878-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66878-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66878-1-PBS cible Nurr1/NR4A2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Nurr1/NR4A2 Protéine recombinante Ag1418
• Nom complet: nuclear receptor subfamily 4, group A, member 2
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC009288
• Symbole du gène: Nurr1
• Identification du gène (NCBI): 4929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

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