Anticorps Monoclonal anti-O-GlcNAc

O-GlcNAc Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgM

Réactivité testée

n/a

Applications

WB

Conjugaison

Non conjugué

CloneNo.

CTD110.6

N° de cat : 65292-1-Ig

Synonymes

glycosylation, O Glc, O-Glc, O-glycosylation, O-linked GlcNAc

---

Galerie de données de validation

• 

  WB analysis using 65292-1-Ig

  Untreated or PUGNAc (an inhibitor of N-acetyl-β-D-glucosaminidase) treated HeLa cells were subjected to SDS PAGE followed by western blot with 65292-1-Ig (O-GlcNAc antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

65292-1-Ig cible O-GlcNAc dans les applications de WB et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: O-GlcNAc
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

O-GlcNAc (O-linked β-N-acetylgulcosamine) is a post-translational modification of serine and threonine residues in proteins. O-GlcNAc modification is abundant in all multicellular eukaryotes, exclusively found on nuclear and cytoplasmic proteins rather than membrane proteins and secretory proteins (PMID: 11269319; 10924527). O-GlcNAc is attached to the protein backbone by enzymatic addition of the N-acetylgulcosamine (GlcNAc) moiety of uridine 5′-diphospho (UDP)-GlcNAc to the hydroxyl oxygen of serines or threonines by the O-linked β-N-acetylgulcosamine transferase (OGT). O-GlcNAc glycosylated proteins can be reversiblely deglycosylated by β-D-N-acetylgulcosaminase (O-GlcNAcase) (PMID: 21526146). O-GlcNAc modification couples many biological processes and plays important roles in development, normal physiology and physiopathology (PMID: 32349769).

{{ptg:RelatedPrimaryAntibodies}}