Anticorps Polyclonal de lapin anti-OAS1/3

• Phare
• Validé par KD/KO

OAS1/3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 14955-1-AP

Synonymes

(2 5)oligo(A) synthase 1, 2 5 oligoadenylate synthase 1, 2 5A synthase 1, E18/E16, IFI 4, OAS1, OAS1/3, OIAS, OIASI, p46/p42 OAS

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Galerie de données de validation

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  WB analysis using 14955-1-AP

  Untreated and IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 14955-1-AP (OAS1/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14955-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14955-1-AP (OAS1/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14955-1-AP (OAS1/3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14955-1-AP (OAS1/3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14955-1-AP (OAS1/3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14955-1-AP (OAS1/3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14955-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14955-1-AP (OAS1/3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: IFN alpha treated HeLa cells, tissu cérébral de souris
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

14955-1-AP cible OAS1/3 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OAS1/3 Protéine recombinante Ag6793
• Nom complet: 2',5'-oligoadenylate synthetase 1, 40/46kDa
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: ~40 kDa, 100-120 kDa
• Numéro d’acquisition GenBank: BC000562
• Symbole du gène: OAS1
• Identification du gène (NCBI): 4938
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009). This antibody may have cross-reaction with OAS3 protein due to the high homology.

Publications

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen ; KO Validated
human		Adv Sci (Weinh); The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.; Authors - Qixue Wang
human	WB	PLoS Biol; Resurrection of 2'-5'-oligoadenylate synthetase 1 (OAS1) from the ancestor of modern horseshoe bats blocks SARS-CoV-2 replication; Authors - Spyros Lytras
mouse	WB	Autophagy; USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.; Authors - Xibao Zhao
human	WB	J Invest Dermatol; OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.; Authors - Yan-Zhou Huang ; KD Validated
human	WB,IHC	Elife; A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.; Authors - Jia Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Seong (Verified Customer) (11-27-2018)

OAS1 polyclonal antibody (14955-1-AP) did work by Western Blot (1:1,500) of ARPE-19 cells induced with interferon gamma but the band was very weak in uninduced cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,500
• Cell Tissue Type: human ARPE-19 cells