Anticorps Polyclonal de lapin anti-OAS2

• Phare
• Validé par KD/KO

OAS2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 19279-1-AP

Synonymes

(2 5)oligo(A) synthase 2, 2 5 oligoadenylate synthase 2, 2 5A synthase 2, OAS2, p69 OAS / p71 OAS, p69OAS / p71OAS

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Galerie de données de validation

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  WB analysis using 19279-1-AP

  IFN alpha treated A549 cells were subjected to SDS PAGE followed by western blot with 19279-1-AP (OAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19279-1-AP

  IP result of anti-OAS2(IP:19279-1-AP, 4ug; Detection:19279-1-AP 1:500) with Jurkat cells lysate 2280 ug.

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  IHC staining of human liver cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19279-1-AP (OAS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19279-1-AP (OAS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19279-1-AP (OAS2 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19279-1-AP (OAS2 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 19279-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OAS2 antibody (19279-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: IFN alpha treated A549 cells,
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

19279-1-AP cible OAS2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OAS2 Protéine recombinante Ag6170
• Nom complet: 2'-5'-oligoadenylate synthetase 2, 69/71kDa
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 66-71 kDa
• Numéro d’acquisition GenBank: BC049215
• Symbole du gène: OAS2
• Identification du gène (NCBI): 4939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are IFN-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS / p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody is specific to isoform p71 and isoform p69 of OAS2.

Publications

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen
human	WB	Nat Commun; Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α; Authors - Adrien Le Thomas
human	IF	Sci Adv; IL-27 signaling activates skin cells to induce innate antiviral proteins and protects against Zika virus infection.; Authors - Jeffery T Kwock
human	WB	Sci Immunol; OAS1/RNase L executes RIG-I ligand-dependent tumor cell apoptosis.; Authors - Daniel F R Boehmer
human,mouse	IF	J Invest Dermatol; Skin Injury Activates A Rapid TRPV1-dependent Antiviral Protein Response.; Authors - Vivian Lei
human	WB	J Invest Dermatol; OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.; Authors - Yan-Zhou Huang ; KD Validated