Anticorps Polyclonal de lapin anti-OBFC2B

• Phare
• Validé par KD/KO

OBFC2B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14809-1-AP

Synonymes

hSSB1, OBFC2B, Sensor of ssDNA subunit B1, SOSS B1, SOSS complex subunit B1, SSB1

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Galerie de données de validation

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  WB analysis using 14809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14809-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14809-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14809-1-AP

  IP Result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IP experiment of HeLa using 14809-1-AP

  IP Result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human ovary tumor using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 14809-1-AP

  Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 14809-1-AP

  Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

14809-1-AP cible OBFC2B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OBFC2B Protéine recombinante Ag6532
• Nom complet: oligonucleotide/oligosaccharide-binding fold containing 2B
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC001079
• Symbole du gène: OBFC2B
• Identification du gène (NCBI): 79035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OBFC2B, also named as NABP2 or SSB1, is a 211 amino acid protein, which belongs to the SOSS-B family. OBFC2B localizes to nuclear foci following DNA damage. OBFC2B is a component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, OBFC2B acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The calculated molecular weight of OBFC2B is 22 kDa, but modified OBFC2B is about 32 kDa.

Publications

Species	Application	Title
human	WB	Cell Res; The Integrator complex controls the termination of transcription at diverse classes of gene targets.; Authors - Jeffrey R Skaar ; KD Validated
human	WB	Cell Death Dis; Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.; Authors - Anaïs Aulas
human	WB,IHC	J Inflamm Res; Comprehensive Analysis of NABP2 as a Prognostic Biomarker and Its Correlation with Immune Infiltration in Hepatocellular Carcinoma; Authors - Bowen Li