Anticorps Polyclonal de lapin anti-OCRL

• Phare
• Validé par KD/KO

OCRL Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 17695-1-AP

Synonymes

INPP5F, LOCR, OCRL, OCRL1

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Galerie de données de validation

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  WB analysis of HeLa using 17695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17695-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17695-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17695-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17695-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17695-1-AP (OCRL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17695-1-AP

  IP result of anti-OCRL (IP:17695-1-AP, 4ug; Detection:17695-1-AP 1:300) with HeLa cells lysate 1080ug.

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  IHC staining of mouse kidney using 17695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17695-1-AP (OCRL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17695-1-AP (OCRL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

17695-1-AP cible OCRL dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OCRL Protéine recombinante Ag11900
• Nom complet: oculocerebrorenal syndrome of Lowe
• Masse moléculaire calculée: 893 aa, 103 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC094726
• Symbole du gène: OCRL
• Identification du gène (NCBI): 4952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OCRL is also named as INPP5F, OCRL1 and belongs to the 5-phosphatase gene family and that Lowe syndrome represents an inborn error of inositol phosphate metabolism(PMID: 9430698).The protein product of the gene that when mutated is responsible for Lowe syndrome, or oculocerebrorenal syndrome (OCRL), is an inositol polyphosphate 5-phosphatase.It may function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes.It has 2 isoforms produced by alternative splicing.Defects in OCRL are the cause of Lowe oculocerebrorenal syndrome (OCRL) and Dent disease type 2 (DD2).This antibody is specific to OCRL.

Publications

Species	Application	Title
human	WB,IF	Mol Autism; Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells.; Authors - Jesse Barnes ; KO Validated
human	WB	Nephrol Dial Transplant; Identification of novel OCRL isoforms associated with phenotypic differences between Dent disease-2 and Lowe syndrome.; Authors - Nana Sakakibara
human	WB	J Neurodev Disord; Transcriptome analysis of neural progenitor cells derived from Lowe syndrome induced pluripotent stem cells: identification of candidate genes for the neurodevelopmental and eye manifestations.; Authors - Hequn Liu ; KD Validated
human	IHC	BMC Med Genomics; Novel pathogenic OCRL mutations and genotype-phenotype analysis of Chinese children affected by oculocerebrorenal syndrome: two cases and a literature review; Authors - Yu Zhang
mouse	WB	Cell Death Dis; Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.; Authors - Viktoriya Zhuravleva ; KD Validated
human	WB,IF	Cell Mol Life Sci; Class I PI3Ks activate stretch-induced autophagy in trabecular meshwork cells; Authors - Myoung Sup Shim

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