Anticorps Recombinant de lapin anti-OCT1

OCT1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243074D12

N° de cat : 85726-1-PBS

Synonymes

NF A1, NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1

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Galerie de données de validation

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  WB analysis using 85726-1-RR (same clone as 85726-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85726-1-RR (OCT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85726-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85726-1-RR (same clone as 85726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85726-1-RR (OCT1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85726-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85726-1-RR (same clone as 85726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85726-1-RR (OCT1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85726-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85726-1-RR (same clone as 85726-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCT1 antibody (85726-1-RR, Clone: 243074D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85726-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85726-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85726-1-RR against Human OCT1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85726-1-PBS cible OCT1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OCT1 Protéine recombinante Ag0586
• Nom complet: POU class 2 homeobox 1
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC003571
• Symbole du gène: POU2F1
• Identification du gène (NCBI): 5451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5'-ATTTGCAT-3'). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].

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