Anticorps Polyclonal de lapin anti-OCT2-Specific

• Phare
• Validé par KD/KO

OCT2-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18996-1-PBS

Synonymes

OCT2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Oct-2, Octamer-binding protein 2

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Galerie de données de validation

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  WB analysis of mouse thymus using 18996-1-AP (same clone as 18996-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 18996-1-AP (same clone as 18996-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18996-1-AP (same clone as 18996-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 18996-1-AP (same clone as 18996-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 18996-1-AP (same clone as 18996-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 18996-1-AP (same clone as 18996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 18996-1-AP (same clone as 18996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18996-1-PBS cible OCT2-Specific dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: POU class 2 homeobox 2
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 52-66 kDa
• Numéro d’acquisition GenBank: NM_002698
• Symbole du gène: POU2F2
• Identification du gène (NCBI): 5452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OCT2, also named as POU2F2 and OTF2, belongs to the POU transcription factor family and Class-2 subfamily. OCT2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). OCT2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. The antibody is specific to OCT2. OCT2 can be detected 52-66 kDa (PMID: 15377492, PMID: 34010659).

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