Anticorps Polyclonal de lapin anti-OCT4/POU5F1

• Phare
• Validé par KD/KO

OCT4/POU5F1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

212

Avis (4)

N° de cat : 11263-1-AP

Synonymes

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

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Galerie de données de validation

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  WB analysis using 11263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 11263-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 11263-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11263-1-AP (OCT4/POU5F1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 11263-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OCT4/POU5F1 antibody (11263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 11263-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OCT4/POU5F1 antibody (11263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of K-562 using 11263-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human OCT4/POU5F1 (11263-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HEK-293T using 11263-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human OCT4/POU5F1 (11263-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7, cellules MDA-MB-231, cellules NCCIT, cellules U-251, tissu cérébral de rat, tissu cérébral de souris, tissu embryonnaire de souris
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu oculaire de souris,
• Résultats positifs en cytométrie: cellules HEK-293T, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 170 publications below
• IP: See 1 publications below
• FC: See 3 publications below

Informations sur le produit

11263-1-AP cible OCT4/POU5F1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OCT4/POU5F1 Protéine recombinante Ag1794
• Nom complet: POU class 5 homeobox 1
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC020712
• Symbole du gène: POU5F1
• Identification du gène (NCBI): 5460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

 What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

 What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing - OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

 What is OCT4's involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).

Publications

Species	Application	Title
human	WB	Mol Cell; Temporal proteomics during neurogenesis reveals large-scale proteome and organelle remodeling via selective autophagy; Authors - Alban Ordureau
mouse		Bone Res; Type II collagen-positive progenitors are important stem cells in controlling skeletal development and vascular formation.; Authors - Xinhua Li
human	WB	Theranostics; Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19.; Authors - Jing Ren
human	WB	Mol Ther; Exosomal miR-1246 from glioma patient body fluids drives the differentiation and activation of myeloid-derived suppressor cells.; Authors - Wei Qiu
mouse	WB	Environ Pollut; Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling; Authors - Yifan Hong
human,mouse	WB	Theranostics; IL20RA signaling enhances stemness and promotes the formation of an immunosuppressive microenvironment in breast cancer.; Authors - Wenjuan Gao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhiping (Verified Customer) (06-21-2023)

This antibody works well for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Udesh (Verified Customer) (11-18-2022)

Worked well in Osteosarcoma U2OS cells. 20ug protein loaded. Dilution 1:2000 in 5% NFDM.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS cells

OCT4/POU5F1 Antibody Western Blot validation (1:2000 dilution) in U2OS cells (Cat no:11263-1-AP)

FH

Gongsheng (Verified Customer) (11-02-2022)

This antibody works well in WB, diluation 1:1000, incubation at +4 °C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

FH

Silvia (Verified Customer) (10-19-2021)

It works well for Immunofluorescence on hiPSCs

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500

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