Anticorps Polyclonal de lapin anti-ODC1

• Phare
• Validé par KD/KO

ODC1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 17003-1-AP

Synonymes

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

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Galerie de données de validation

• 

  WB analysis of rat thymus using 17003-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 17003-1-AP (ODC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17003-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17003-1-AP (ODC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 17003-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17003-1-AP (ODC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17003-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17003-1-AP (ODC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17003-1-AP

  Immunofluorescent analysis of HepG2 cells, using ODC1 antibody 17003-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IHC: tissu d'hyperplasie de la prostate humain, tissu de cancer de la prostate humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Informations sur le produit

17003-1-AP cible ODC1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ODC1 Protéine recombinante Ag10699
• Nom complet: ornithine decarboxylase 1
• Masse moléculaire calculée: 461 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC025296
• Symbole du gène: ODC1
• Identification du gène (NCBI): 4953
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.

Publications

Species	Application	Title
human	IHC	NPJ Precis Oncol; Integrated multiomics analysis of hepatoblastoma unravels its heterogeneity and provides novel druggable targets; Authors - Masahiro Sekiguchi
human	IHC	NPJ Precis Oncol; Integrated multiomics analysis of hepatoblastoma unravels its heterogeneity and provides novel druggable targets.; Authors - Masahiro Sekiguchi ; KD Validated
mouse	WB	Am J Physiol Cell Physiol; The Regulation of Polyamine Pathway Proteins in Models of Skeletal Muscle Hypertrophy and Atrophy - a potential role for mTORC1.; Authors - Michael Tabbaa
human	WB	Life Sci; Ornithine is a key mediator in hyperphosphatemia-mediated human umbilical vein endothelial cell apoptosis: Insights gained from metabolomics.; Authors - Rong Zhou
human	WB	Arch Pharm Res; Spermidine rescues proximal tubular cells from oxidative stress and necrosis after ischemic acute kidney injury.; Authors - Jinu Kim ; KO Validated
mouse	WB	Anat Cell Biol; PMID: 30310711; Authors - Sang Pil Yoon ; KD Validated