Anticorps Recombinant de lapin anti-ODF2

ODF2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251271E12

N° de cat : 86459-2-RR

Synonymes

Cenexin, ODF2/1

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Galerie de données de validation

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  WB analysis using 86459-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86459-2-RR (ODF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86459-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86459-2-RR against Human ODF2 were performed. The affinity constant is 0.153 nM.

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Applications testées

• Résultats positifs en WB: OVCAR-3 cells, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86459-2-RR cible ODF2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ODF2 Protéine recombinante Ag2693
• Nom complet: outer dense fiber of sperm tails 2
• Masse moléculaire calculée: 763 aa, 89 kDa
• Poids moléculaire observé: 80-100 kDa
• Numéro d’acquisition GenBank: BC010629
• Symbole du gène: ODF2
• Identification du gène (NCBI): 4957
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.

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