Anticorps Polyclonal de lapin anti-Teneurin 1

Teneurin 1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 21696-1-AP

Synonymes

ODZ1, ODZ3, Protein Odd Oz/ten m homolog 1, Ten 1, TEN M1, Tenascin M1, Teneurin 1, Teneurin-1, TNM, TNM1

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Galerie de données de validation

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  WB analysis of H69/H146 cells using 21696-1-AP

  WB result of anti-ODZ1 (21696-1-AP) with two cell lines by Dr. Petrini, Iacopo.

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  WB analysis using 21696-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21696-1-AP (ODZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21696-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21696-1-AP (ODZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21696-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21696-1-AP (ODZ1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21696-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21696-1-AP (ODZ1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21696-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21696-1-AP (ODZ1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21696-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21696-1-AP (Teneurin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21696-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21696-1-AP (Teneurin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules H69/H146, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

21696-1-AP cible Teneurin 1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Teneurin 1 Protéine recombinante Ag16289
• Nom complet: odz, odd Oz/ten-m homolog 1(Drosophila)
• Masse moléculaire calculée: 2732 aa, 306 kDa
• Poids moléculaire observé: 305 kDa
• Numéro d’acquisition GenBank: BC140783
• Symbole du gène: Teneurin 1
• Identification du gène (NCBI): 10178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ODZ1 (also known as teneurin-1, TENM1), which plays a key role in central nervous system ontogenesis. ODZ1 is upregulated in Glioblastoma cells through a Stat3-mediated pathway activated by IL-6, which is released by tumor-associated monocytes. In addition, a hypoxic microenvironment regulates GBM tumor cell migration in part by inducing ODZ1 through hypomethylation of a CpG island in the ODZ1 promoter.

Publications

Species	Application	Title
human	WB	Nature; The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.; Authors - Barretina Jordi J
human	WB	Genet Res (Camb); Impact of Extracellular Matrix-Related Genes on the Tumor Microenvironment and Prognostic Indicators in Esophageal Cancer: A Comprehensive Analytical Study; Authors - Yinghong Wu

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