Anticorps Monoclonal anti-OGDH

OGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H7A2

N° de cat : 66285-1-PBS

Synonymes

AKGDH, E1k, OGDC, OGDC E1, OGDH

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Galerie de données de validation

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  WB analysis of human heart using 66285-1-Ig (same clone as 66285-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66285-1-Ig (same clone as 66285-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66285-1-Ig (same clone as 66285-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OGDH antibody (66285-1-Ig, Clone: 1H7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66285-1-PBS cible OGDH dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OGDH Protéine recombinante Ag7484
• Nom complet: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Masse moléculaire calculée: 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC004964
• Symbole du gène: OGDH
• Identification du gène (NCBI): 4967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.

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