Anticorps Recombinant de lapin anti-OGDH

• Phare
• Validé par KD/KO

OGDH Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C16

N° de cat : 82773-1-PBS

Synonymes

1C16, 2-oxoglutarate dehydrogenase complex component E1, 2-oxoglutarate dehydrogenase, mitochondrial, Alpha-ketoglutarate dehydrogenase, Alpha-KGDH-E1

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Galerie de données de validation

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  WB analysis using 82773-1-RR (same clone as 82773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82773-1-RR (same clone as 82773-1-PBS)

  WB result of OGDH antibody (82773-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OGDH transfected HeLa cells. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 82773-1-RR (same clone as 82773-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using OGDH antibody (82773-1-RR, Clone: 1C16 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 82773-1-RR (same clone as 82773-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using OGDH antibody (82773-1-RR, Clone: 1C16 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82773-1-PBS cible OGDH dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OGDH Protéine recombinante Ag7368
• Nom complet: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Masse moléculaire calculée: 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC004964
• Symbole du gène: OGDH
• Identification du gène (NCBI): 4967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

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