Anticorps Recombinant de lapin anti-OGDHL

OGDHL Recombinant Antibody for IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230228B2

N° de cat : 82981-1-PBS

Synonymes

230228B2, 2-oxoglutarate dehydrogenase complex component E1-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, Alpha-ketoglutarate dehydrogenase-like, EC:1.2.4.2

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Galerie de données de validation

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  IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 82981-1-RR (same clone as 82981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

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  FC experiment of Caco-2 using 82981-1-RR (same clone as 82981-1-PBS)

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug OGDHL Recombinant antibody (82981-1-RR, Clone:230228B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82981-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82981-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82981-1-RR against Human OGDHL were performed. The affinity constant is 4.98 nM.

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Informations sur le produit

82981-1-PBS cible OGDHL dans les applications de IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OGDHL Protéine recombinante Ag10794
• Nom complet: oxoglutarate dehydrogenase-like
• Masse moléculaire calculée: 1010 aa, 115 kDa
• Numéro d’acquisition GenBank: BC026320
• Symbole du gène: OGDHL
• Identification du gène (NCBI): 55753
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.

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