Anticorps Polyclonal de lapin anti-OGFOD1

OGFOD1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30800-1-PBS

Synonymes

2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1, EC:1.14.11.-, KIAA1612, OGFOD 1, Prolyl 3-hydroxylase OGFOD1

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Galerie de données de validation

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  WB analysis using 30800-1-AP (same clone as 30800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30800-1-AP (OGFOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30800-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 30800-1-AP (same clone as 30800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30800-1-AP (OGFOD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30800-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30800-1-PBS cible OGFOD1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OGFOD1 Protéine recombinante Ag34034
• Nom complet: 2-oxoglutarate and iron-dependent oxygenase domain containing 1
• Masse moléculaire calculée: 542 aa, 63 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC032919
• Symbole du gène: OGFOD1
• Identification du gène (NCBI): 55239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1 (OGFOD1) is a proly hydroxylase which belongs to 2-OG-Fe (II) dioxygenase family, it also namely KIAA1612 and TPA1. OGFOD1 is expressed in many tissues, mainly located in the nucleoplasm. It increased in many cancers and regulates both transcription and translation. The apparent molecular weight is larger than the calculated molecular weight of 63 kDa, which is likely due to posttranslational modification, presumably by glycosylation. (PMID:34298635 and 20579638)

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