Anticorps Polyclonal de lapin anti-OGFR

• Phare
• Validé par KD/KO

OGFR Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 11177-1-AP

Synonymes

OGFR, opioid growth factor receptor, Protein 7 60, Zeta type opioid receptor

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Galerie de données de validation

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  WB analysis of COLO 320 using 11177-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11177-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11177-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11177-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 11177-1-AP

  IP Result of anti-OGFR (IP:11177-1-AP, 5ug; Detection:11177-1-AP 1:500) with COLO 320 cells lysate 1200ug.

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  IHC staining of human colon cancer using 11177-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11177-1-AP (OGFR antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of COLO 320 using 11177-1-AP

  Immunofluorescent analysis of COLO 320 cells using 11177-1-AP (OGFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of L02 using 11177-1-AP

  Immunofluorescent analysis of L02 cells using 11177-1-AP (OGFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 11177-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug OGFR antibody (11177-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules A549, cellules HEK-293, cellules L02
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules COLO 320, cellules L02
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 10 publications below
• IF: See 1 publications below

Informations sur le produit

11177-1-AP cible OGFR dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OGFR Protéine recombinante Ag1671
• Nom complet: opioid growth factor receptor
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 90-110 kDa
• Numéro d’acquisition GenBank: BC014137
• Symbole du gène: OGFR
• Identification du gène (NCBI): 11054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; CIRBP-OGFR axis safeguards against cardiomyocyte apoptosis and cardiotoxicity induced by chemotherapy.; Authors - Cihang Liu ; KD Validated
human	IHC	Front Oncol; Opioid Receptor Expression in Colorectal Cancer: A Nested Matched Case-Control Study.; Authors - Amparo Belltall
human	IHC	Int Immunopharmacol; Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor.; Authors - Na Qu
human	WB, IHC	Int Immunopharmacol; Methionine enkephalin activates autophagy and stimulates tumour cell immunogenicity in human cutaneous squamous cell carcinoma.; Authors - Xueli Bai
human	WB, IHC, IF	Int Immunopharmacol; Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma.; Authors - Xueli Bai
human	WB,IHC	Int Immunopharmacol; A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment.; Authors - Shuling Zhang ; KD Validated