Anticorps Recombinant de lapin anti-OGFR

OGFR Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250452E7

N° de cat : 85998-1-PBS

Synonymes

Opioid growth factor receptor, Protein 7 60, Protein 7-60, Zeta type opioid receptor, Zeta-type opioid receptor

---

Galerie de données de validation

• 

  WB analysis using 85998-1-RR (same clone as 85998-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85998-1-RR (OGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

• 

  IP experiment of COLO 320 using 85998-1-RR (same clone as 85998-1-PBS)

  IP result of anti-OGFR (IP:85998-1-RR, 4ug; Detection:85998-1-RR 1:3000) with COLO 320 cells lysate 1920 ug. This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 85998-1-RR (same clone as 85998-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85998-1-RR (OGFR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 85998-1-RR (same clone as 85998-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85998-1-RR (OGFR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

• 

  IF Staining of L02 using 85998-1-RR (same clone as 85998-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using OGFR antibody (85998-1-RR, Clone: 250452E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85998-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85998-1-RR against Human OGFR were performed. The affinity constant is 0.211 nM.

---

Informations sur le produit

85998-1-PBS cible OGFR dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OGFR Protéine recombinante Ag1671
• Nom complet: opioid growth factor receptor
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC014137
• Symbole du gène: OGFR
• Identification du gène (NCBI): 11054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}