Anticorps Polyclonal de lapin anti-OGG1

OGG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(36)

Avis (3)

N° de cat : 15125-1-AP

Synonymes

8 oxoguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase, Ogg 1

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Galerie de données de validation

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  WB analysis using 15125-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15125-1-AP (OGG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 15125-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15125-1-AP (OGG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15125-1-AP

  IP result of anti-OGG1 (IP:15125-1-AP, 4ug; Detection:15125-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of mouse liver using 15125-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15125-1-AP (OGG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15125-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15125-1-AP (OGG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15125-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using OGG1 antibody (15125-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293 transfectées, cellules HeLa, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 31 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

15125-1-AP cible OGG1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OGG1 Protéine recombinante Ag7204
• Nom complet: 8-oxoguanine DNA glycosylase
• Masse moléculaire calculée: 22 kDa, 36-40 kDa, 45-57 kDa
• Poids moléculaire observé: 36-40 kDa, 45-50 kDa
• Numéro d’acquisition GenBank: BC000657
• Symbole du gène: OGG1
• Identification du gène (NCBI): 4968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base by product that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa). Our OGG1 antibody detects all the isoforms. We always got the strongest 47 kDa corresponds to isoform Beta in our detection and some weaker bands (with long time exposure). The expression amount of Beta is higher than other isoforms from our data. This antibody has been cited in more than 4 publications, WB and IHC detection in mouse and human.

Publications

Species	Application	Title
mouse	IHC	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
human	WB	Redox Biol; Oxidative damage to lung mitochondrial DNA is a key contributor to the development of chemical lung injury; Authors - Shubham Dubey
mouse	WB	PLoS Genet; Heterozygosity for a hypomorphic polβ mutation reduces the expansion frequency in a mouse model of the fragile x-related disorders.; Authors - Rachel Adihe Lokanga
mouse	WB	JHEP Rep; TAK1 deficiency promotes liver injury and tumorigenesis via ferroptosis and macrophage cGAS-STING signalling; Authors - Wantong Su
mouse	WB	Phytomedicine; Nrf2-dependent hepatoprotective effect of ellagic acid in titanium dioxide nanoparticles-induced liver injury; Authors - Kunpeng Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shubhangi (Verified Customer) (07-05-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: Ac16

OGG1 Antibody Western Blot validation (1000 dilution) in Ac16 (Cat no:15125-1-AP)

FH

s (Verified Customer) (12-12-2022)

very good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mouse lung

OGG1 Antibody Western Blot validation (1000 dilution) in mouse lung (Cat no:15125-1-AP)

FH

Ruyi (Verified Customer) (08-19-2019)

This antibody works well in my WB experiments.

• Applications: Western Blot,

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