Anticorps Recombinant de lapin anti-OGG1

OGG1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240940H3

N° de cat : 83973-5-RR

Synonymes

240940H3, 8 oxoguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase

---

Galerie de données de validation

• 

  WB analysis using 83973-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83973-5-RR (OGG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 83973-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using OGG1 antibody (83973-5-RR, Clone: 240940H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 83973-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83973-5-RR against Human OGG1 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique de porc, tissu de muscle squelettique de rat
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83973-5-RR cible OGG1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: OGG1 Protéine recombinante Ag7204
• Nom complet: 8-oxoguanine DNA glycosylase
• Masse moléculaire calculée: 22 kDa, 36-40 kDa, 45-57 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC000657
• Symbole du gène: OGG1
• Identification du gène (NCBI): 4968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base by product that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa).

{{ptg:RelatedPrimaryAntibodies}}