Anticorps Monoclonal anti-Osteoglycin

Osteoglycin Monoclonal Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

2A5F11

N° de cat : 66382-1-PBS

Synonymes

OGN, Osteoinductive factor, OIF, OG, Mimecan

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Galerie de données de validation

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  WB analysis of human artery using 66382-1-Ig (same clone as 66382-1-PBS)

  Human artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  WB analysis of pig artery using 66382-1-Ig (same clone as 66382-1-PBS)

  Pig artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50723-1

  Sandwich ELISA standard curve of MP50723-1, Human Osteoglycin Monoclonal Matched Antibody Pair - PBS only. 66382-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3484. 66382-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP50723-1

  Plasma of six individual healthy human donors was measured. The Osteoglycin concentration of detected samples was determined to be 1.7 ng/mL with a range of 1.2-2.2 ng/mL.

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  Sample test of MP50723-1

  Serum of six individual healthy human donors was measured. The Osteoglycin concentration of detected samples was determined to be 1.9 ng/mL with a range of 1.4-2.3 ng/mL.

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  IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50723-1

  Cytometric bead array standard curve of MP50723-1, OGN/Osteoglycin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66382-1-PBS. Detection antibody: 66382-2-PBS. Standard:Ag3484. Range: 0.098-100 ng/mL.

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Informations sur le produit

66382-1-PBS cible Osteoglycin dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Osteoglycin Protéine recombinante Ag3484
• Nom complet: osteoglycin
• Masse moléculaire calculée: 298 aa, 34 kDa
• Poids moléculaire observé: 30-40 kDa
• Numéro d’acquisition GenBank: BC037273
• Symbole du gène: OGN
• Identification du gène (NCBI): 4969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

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